ABSTRACT
Objective: In this study, nano-biocomposite composed of poly [lactide-co-glycolide] [PLGA] and chitosan [CS] were electrospun through a single nozzle by dispersing the CS nano-powders in PLGA solution. The cellular behavior of human adipose derived stem cells [h-ADSCs] on random and aligned scaffolds was then evaluated
Materials and Methods: In this experimental study, the PLGA/CS scaffolds were prepared at the different ratios of 90/10, 80/20, and 70/30 [w/w]%. Morphology, cell adhesion and proliferation rate of h-ADSCs on the scaffolds were assessed using scanning electron microscope [SEM], 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide [MTT] assay and trypan blue staining respectively
Results: H-ADSCs seeded on the matrices indicated that the PLGA/CS composite matrix with aligned nanofibres and higher content of CS nano-powders gave significantly better performance than others in terms of cell adhesion and proliferation rate [P<0.05]
Conclusion: We found that CS enhanced cell adhesion and proliferation rate, and aligned nanofibers guided cell growth along the longitudinal axis of the nanofibers, which would provide a beneficial approach for tissue engineering